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KMID : 0379119870150010019
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Korean Journal of Mycology
1987 Volume.15 No. 1 p.19 ~ p.22
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Protoplast Isolation and reversion from Pleurotus spodoleucus
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Abstract
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This experiment was undertaken to investigate proper conditions for protoplast formation from Pleurotua apodoleucus. Novozym 234 was the moat effective enzyme and a high yield of protoplasta was obtained. Combination of enzymes did not improve this result. Sucrose gave the best result to support the release and maintain the stability of protoplasts. The optimal reaction time of mycelium with the lytic mixture was 3 hrs. in shaking condition at 120 strokes min^(-1). When the mycelium of P. spodoleucus was cultured for 3 days on mushroom complete agar medium at 27℃, the formation of protoplasta was effective. The mushroom complete agar medium stabilized with 0.6 M sucrose was the most effective for reversion of protoplasta.
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